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Custom Protein Production Service

 

Gemini Biosciences can produce recombinant proteins to customers’ specifications from vectors supplied by them or produced by us as part of the DNA cloning service.

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The general workflow includes:
 

  • Transformation or transfection of cells with expression construct e.g. plasmid DNA or virus construct

  • Identification and selection of over-expressing colonies or stable cell lines

  • Small-scale expression and purification and assessment using SDS-PAGE

  • Optimization of protein expression parameters

  • Western blot (anti-tag) analysis of expressed protein if yield is low

  • Estimation of protein yield

  • Scale up growth of over-expressing strain s

  • Cell harvesting and lysis

  • Purification of protein from cleared lysate via purification tag or customer's preferred system

  • SDS-PAGE or Western blot analysis of purified protein using anti expression tag antibody

  • Determination of protein concentration

  • Delivery of purified protein  to customer

 

When required by the client, we can produce the recombinant protein on a large-scale following establishment of protocols.

 

If you are interested in generating recombinant proteins for any purpose, please contact us for details of our process and a quote.

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