Publications

The publicatons provided below are from the Gemini Biosciences team in the technologies being commercialised by the company

Proteomics, Cell biology, Molecular biology

 

PROTEOMICS, MOLECULAR & CELL BIOLOGY

  • Alqaseer K, Turapov O, Barthe P, Jagatia H, De Visch A, Roumestand C, Wegrzyn M, Bartek IL, Voskuil MI, O'Hare HM, Ajuh P, Bottrill AR, Witney AA, Cohen-Gonsaud M, Waddell SJ, Mukamolova GV. (2019) Protein kinase B controls Mycobacterium tuberculosis growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112. Mol. Microbiol. [Epub ahead of print]

  • Iswahyudi, Mukamolova GV, Straatman-Iwanowska AA, Allcock N, Ajuh P, Turapov O, O'Hare HM. (2019) Mycobacterial phosphatase PstP regulates global serine threonine phosphorylation and cell division. Sci. Rep. 9(1):8337. 

  • Turapov O, Forti F, Kadhim B, Ghisotti D, Sassine J, Straatman-Iwanowska A, Bottrill AR, Moynihan PJ, Wallis R, Barthe P, Cohen-Gonsaud M, Ajuh P, Vollmer W, Mukamolova GV.(2018)Two Faces of CwlM, an Essential PknB Substrate, in Mycobacterium tuberculosis. J. Cell Rep. 25(1):57-67  

  • Cui L, Rashdan NA, Zhu D, Milne EM, Ajuh P, Milne G, Helfrich MH, Lim K, Prasad S, Lerman DA, Vesey AT, Dweck MR, Jenkins WS, Newby DE, Farquharson C, Macrae VE.(2017) End stage renal disease-induced hypercalcemia may promote aortic valve calcification via Annexin VI enrichment of valve interstitial cell derived-matrix vesicles. J. Cell Physiol. 232(11):2985-2995.

  • Stebbing, J., Zhang, H., Xu, Y., Grothey, A., Ajuh, P., Angelopoulos, N., and Giamas, G. (2015) Reprogramming of the tyrosine kinase-regulated proteome in breast cancer by combined use of RNAi and SILAC quantitative proteomics. Mol. Cell. Proteomics. 14(9): 2479-2492

  • Enayetallah A, Nadanaciva S, Ajuh P, Vázquez-Martín C, Tavendale A, Lamond A, Ziemek D and Leach KL (2015) Assessing Hepatotoxicants based on High-throughput Quantitative SILAC Proteomics and Causal Biological Networks, J. Proteomics Bioinform., Vol. 8.3 pp. 049-059

  • Li J, Hou B, Tumova S, Muraki K, Bruns A, Ludlow MJ, Sedo A, Hyma AJ, McKeown L, Young RS, Yuldasheva NY, Majeed Y, Wilson LA, Rode B, Bailey MA, Fu Z, Carter DA, Bilton J, Imrie H, Ajuh P, Dear TN, Cubbon RM, Kearney MT, Prasad RK, Evans PC, Ainscough JF, Beech DJ (2014) Piezo1 integration of vascular architecture with physiological force. Nature vol. 515 (7526) pp. 279-282.

  • Babaei F, Ramalingam R, Tavendale A, Liang Y, Yan LS, Ajuh P, Cheng SH, Lam YW. (2013) Novel Blood Collection Method Allows Plasma Proteome Analysis from Single Zebrafish J. Proteome Res. 12 (4): 1580-90.

  • Löscher M, Schosserer M, Dausse E, Lee K, Ajuh P, Grillari-Voglauer R, Lamond AI, Toulmé JJ, Grillari J. (2012) Inhibition of pre-mRNA splicing by a synthetic Blom7α-interacting small RNA. PLoS One. 7(10): e47497.

  • Cai X, Ramalingam R, Wong HS, Cheng J, Ajuh P, Cheng SH, Lam YW.  (2012) Characterization of carbon nanotube-protein corona by using quantitative proteomics. Nanomedicine. 9 (5) 583-593.

  • Dove BK, Surtees R, Bean TJ, Munday D, Wise HM, Digard P, Carroll MW, Ajuh P, Barr JN, Hiscox JA (2012) A quantitative proteomic analysis of lung epithelial (A549) cells infected with 2009 pandemic influenza A virus using stable isotope labeling with amino acids in cell culture. Proteomics. 12(9): 1431-6.

  • Dellago H, Löscher M, Ajuh P, Ryder U, Kaisermayer C, Grillari-Voglauer R, Fortschegger K, Gross S, Gstraunthaler A, Borth N, Eisenhaber F, Lamond AI, Grillari J. (2011) Exo70, a subunit of the exocyst complex, interacts with SNEV(hPrp19/hPso4) and is involved in pre-mRNA splicing. Biochem J. 438(1):81-91.

  • Wagner W, Ajuh P, Löwer J, Wessler S. (2011) Quantitative phosphoproteomic analysis of prion-infected neuronal cells. Cell Commun Signal. 8:28.

  • Llères D, Denegri M, Biggiogera M, Ajuh P, Lamond AI. (2010) Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice. EMBO Rep. 11(6): 445-51.

  • Emmott E, Rodgers MA, Macdonald A, McCrory S, Ajuh P, Hiscox JA. (2010) Quantitative proteomics using stable isotope labeling with amino acids in cell culture reveals changes in the cytoplasmic, nuclear, and nucleolar proteomes in Vero cells infected with the coronavirus infectious bronchitis virus. Mol Cell Proteomics. 9(9): 1920-36.

  • Grillari J, Löscher M, Denegri M, Lee K, Fortschegger K, Eisenhaber F, Ajuh P, Lamond AI, Katinger H, Grillari-Voglauer R (2009) Blom7alpha is a novel heterogeneous nuclear ribonucleoprotein K homology domain protein involved in pre-mRNA splicing that interacts with SNEVPrp19-Pso4. J Biol Chem. 284(42): 29193-204.

  • Bracken CP, Wall SJ, Barré B, Panov KI, Ajuh P, Perkins ND. (2008)Regulation of cyclin D1 RNA stability by SNIP1.  Cancer Res. 68(18):7621-8.

  • Grillari J, Ajuh P, Stadler G, Löscher M, Voglauer R, Ernst W, Chusainow J, Eisenhaber F, Pokar M, Fortschegger K, Grey M, Lamond AI, Katinger H. (2005) SNEV is an evolutionarily conserved splicing factor whose oligomerization is necessary for spliceosome assembly. Nucleic Acids Res. 33(21): 6868-83.

  • Chusainow J, Ajuh P, Trinkle-Mulcahy L, Sleeman JE, Ellenberg J, Lamond AI.  (2005) FRET analyses of the U2AF complex localize the U2AF35/U2AF65 interaction in vivo and reveal a novel self-interaction of U2AF35. RNA. 11(8):1201-14.

  • Löscher M, Fortschegger K, Ritter G, Wostry M, Voglauer R, Schmid JA, Watters S, Rivett AJ, Ajuh P, Lamond AI, Katinger H, Grillari J. (2005) Interaction of U-box E3 ligase SNEV with PSMB4, the beta7 subunit of the 20 S proteasome. Biochem J. 388(Pt 2): 593-603.

  • Ajuh P and Lamond AI. (2003) Identification of peptide inhibitors of pre-mRNA splicing derived from the essential interaction domains of CDC5L and PLRG1. Nucleic Acids Res. 31(21): 6104-16.

  • Leonard D, Ajuh P, Lamond AI, Legerski RJ. (2003) hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing. Biochem Biophys Res. Commun. 308(4): 793-801.

  • Ajuh P, Chusainow J, Ryder U, Lamond AI. (2002) A novel function for human factor C1 (HCF-1), a host protein required for herpes simplex virus infection, in pre-mRNA splicing. EMBO J. 21(23): 6590-602.

  • Sleeman JE, Ajuh P, Lamond AI. (2001) snRNP protein expression enhances the formation of Cajal bodies containing p80-coilin and SMN. J Cell Sci. 114 (Pt 24): 4407-19.

  • Ajuh P, Sleeman J, Chusainow J, Lamond AI. (2001) A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing. J Biol Chem.  276(45): 42370-81.

  • Rappsilber J, Ajuh P, Lamond AI, Mann M. (2001) SPF30 is an essential human splicing factor required for assembly of the U4/U5/U6 tri-small nuclear ribonucleoprotein into the spliceosome. J Biol Chem. 276(33): 31142-50.

  • Skalsky YM, Ajuh P, Parker C, Lamond AI, Goodwin G, Cooper CS. (2001) PRCC, the commonest TFE3 fusion partner in papillary renal carcinoma is associated with pre-mRNA splicing factors. Oncogene. 20(2): 178-87.

  • Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI. (2000) Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry. EMBO J. 19(23): 6569-81.

  • Yue BG, Ajuh P, Akusjärvi G, Lamond AI, Kreivi JP. (2000) Functional coexpression of serine protein kinase SRPK1 and its substrate ASF/SF2 in Escherichia coli. Nucleic Acids Res. 28(5): E14.

  • Ajuh P, Browne GJ, Hawkes NA, Cohen PT, Roberts SG, Lamond AI. (2000) Association of a protein phosphatase 1 activity with the human factor C1 (HCF) complex. Nucleic Acids Res. 28(3): 678-86.

  • Trinkle-Mulcahy L, Ajuh P, Prescott A, Claverie-Martin F, Cohen S, Lamond AI, Cohen P. (1999) Nuclear organisation of NIPP1, a regulatory subunit of protein phosphatase 1 that associates with pre-mRNA splicing factors. J Cell Sci. 112 ( Pt 2): 157-68.

  • Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M. (1998) Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex. Nat Genet. 20(1): 46-5

EXTRACELLULAR VESICLES ANALYSIS

  • Simon, T., Pinioti, S., Schellenberger, P., Rajeeve, V., Wendler, F., Cutillas, PR., King, A., Stebbing, J., Giamas, G. Shedding of bevacizumab in tumour cells-derived extracellular vesicles as a new therapeutic resistance mechanism in glioblastoma. 2018. (Molecular Cancer; in press)

  • Gagliano, T., Simon, T., Giamas, G. miRNA transported by exosomes: a key machinery in tumor microenvironment mediated chemoresistance. 2016. Translational Cancer Research.

  • Wendler, F., Favichio, R., Simon, T., Alifranchis, C., Stebing, J., Giamas, G. Extracellular vesicles swarm the cancer microenvironment: From tumor-stroma communication to drug intervention. 2016. Oncogene. 

  • Wendler, F., Stamp, G., Giamas, G. Tumor-stromal cell communication: small vesicles signal big changes. 2016. Trends in Cancer.

  • Elliott PR, Irvine AF, Jung HS, Tozawa K, Pastok MW, Picone R, Badyal SK, Basran J, Rudland PS, Barraclough BR, Lian LY, Bagshaw CR, Kriajevska M and Barsukov IL (2012) Novel mode of Ca2+-S100A4 interaction with non-muscle myosin IIA heavy chain generates nanomolar affinity required for filament remodelling, Structure, 20:654-666.

  • Herbert AP, Riesen M, Bloxam L, Kosmidou E, Wareing BM, Johnson JR,. Pennington SR, Lian LY, and Morgan A, (2012) Hsp12: NMR structure and identification of a role in rDNA recombination and dietary restriction-induced lifespan extension, PLoS ONE 7(7): e41975. doi:10.1371/journal.pone.0041975

  • Patel P, Clarke C, Barraclough DL, Rudland PS, Barraclough BR and Lian LY (2013) Structure of the anterior-gradient 2 proteins reveals a dimeric thioredoxin fold with a cell adhesion role, J. Mol. Biol. 425: 929-943

  • Berry, J-L, Phelan M, Collins RF, Adomavicius T, Tonjum T, Frye SA, Bird L, Owens R, Ford RC, Lian LY and Derrick JP (2012) Structure and Assembly of a Trans-Periplasmic Channel for Type IV Pili in Neisseria meningitidis. PLoS Pathog 8(9): e1002923. doi:10.1371/journal.ppat.1002923

  • McCue HV, Patel P, Herbert AP, Lian LY, Burgoyne RD, and Haynes LP (2012) Solution NMR Structure of the Ca2+-Bound N-Terminal Domain of Calcium Binding Protein 7 (CaBP7): A regulator of Golgi trafficking J. Biol. Chem. 287: Issue: 45 DOI: 10.1074/jbc.M112.402289

  • Elliott, VL, Edge, GT, Phelan, MM, Lian, LY, Webster, R, Finn, RF, Park, BK, Kitteringham, NR (2012) Evidence for Metabolic Cleavage of a PEGylated Protein in Vivo Using Multiple Analytical Methodologies Molecular Pharmaceutics, 9: 1291-1301.

  • Tang, W J, Johnston, S, Iggo, JA, Berry, NG, Phelan, M, Lian, LY, Bacsa, J., and Xiao, J. L. (2013) Angewandte Chemie-International Edition 52, 1668-1672

  • Vogrig A, Dorr L, Bouzidi N, Boucherle B, Wattiez AS, Cassier E, Vallon G, Ripoche I, Abrunhosa-Thomas I, Marin P, Nauton L, Thery V, Courteix C, Lian LY and Ducki S, (2013) Structure-Based Design of PDZ Ligands as Inhibitors of 5-HT2A Receptor / PSD-95 PDZ1 Domain Interaction Possessing Anti-Hyperalgesic Activity, ACS Chem Biol, 8: 2209-2216

  • Bouzidi N, Deokar H,Vogrig A, Boucherle B, Ripoche I ,Abrunhosa-Thomas I, Dorr L, Wattiez AS , Lian LY, Marin P , Courteix, C , Ducki, S (2013) Identification of PDZ ligands by docking-based virtual screening for the development of novel analgesic agents, Bioorganic and Medicinal Chemistry Letts, 23:2624-2627

  • Lian LY (2013) NMR studies of weak protein-protein interactions, Prog. NMR Spectr. 71: 59-72

  • Carnell, AJ, Kirk, R, Smith M, McKenna S, Lian LY and Gibson R, (2013) Inhibition of Human alpha-Methylacyl CoA Racemase (AMACR): a Target for Prostate Cancer, ChemMedChem, 8:1643-1647

  • Yao, S, Ireland, SJ, Bee, A, Beesley, C, Forootan, SS, Dodson, A, Dickinson, T, Gerard, P, Lian, LY, Risk, JM, Smith, P, Malki, MI, Ke, Y, Cooper, CS, Gosden, C, Foster, CS (2012) Splice variant PRKC-zeta-(PrC) is a novel biomarker of human prostate cancer British Journal of Cancer, 107:388-399.

  • Awais, M, Javed, M A, Wen, L, Gibson, R, Shore, E, Kershaw, N, Criddle, DN., Tepikin, A, Berry, N, O'Neill, P, Lian, LY, Park, BK and Sutton, R (2013) Development of Small Molecule Inhibitors of Cyclophilin D for Treatment of Severe Acute Pancreatitis Pancreas 42, 1338-1338

  • Lian, LY, Pandalaneni SR, Todd, PAC, Martin VM, Burgoyne RD, and Haynes, LP (2014) Demonstration of Binding of Neuronal Calcium Sensor•1 to the Cav2.1 P/Q-Type Calcium Channel Biochemistry, 53, 6052•6062

  • Phelan M M, McLean L, Hurst J L, Beynon R J and Lian LY (2014) Comparative study of the molecular variation between 'central' and 'peripheral' MUPs and significance for behavioral signaling (2014), Biochem Transaction, 42, 866-872

  • Phelan M M, McLean L, Simpson D M, Hurst J L, Beynon R J and Lian LY (2014) Structural insights into the specificity of darcin, an atypical major urinary protein. PLOS One 9:e108415

Structural biology, Metabonomics & Biophysical analyses

Find us

Gemini Biosciences

131 Mount Pleasant

Liverpool Science Park

Liverpool

L3 5TF

United Kingdom

 

t: +44 (0) 151 230 2265 

e: orders@geminibioscience.com 

    info@geminibioscience.com 

Copyright © 2015 Gemini Biosciences Ltd