PRODUCTS AND SERVICES
2: CUSTOM ANTIBODY PRODUCTION
2.4 Production of ascites
In some cases, in vitro production of monoclonal antibodies may not meet the scientific aims of a project therefore mouse ascites may be required for the following reasons:
Some hybridoma cell lines do not adapt well to in vitro conditions.
Monoclonal antibodies from mouse ascitic fluids might be essential for experiments in which mAb are used in mice.
Rat hybridoma cell lines do not generate ascites efficiently in rats, usually adapt poorly to in vitro conditions, but usually generate ascites in immunocompromised mice.
Downstream purification can lead to protein denaturation and decreased antibody activity.
Serum-free or low-serum conditions cannot provide sufficient amounts of mAb for some purposes, such as the evaluation of new vaccines against infectious organisms.
Culture methods sometimes yield populations of IgG mAb that are glycosylated at positions different from those harvested from mouse ascites fluid, thereby influencing antigen-binding capacity and important biologic functions.
When hybridoma cells producing mAb are contaminated with infectious agents, such as yeasts or fungi, the cells often must be passed through mice.
Therefore, upon development of the customer’s monoclonal antibody, Gemini Biosciences can produce high monoclonal antibody titre mouse ascites for use in the customer’s research applications. We can also produce ascites from a customer's hybridoma cell line expressing their mAB of interest.
Please contact us for a quote and details on our antibody development process.