PRODUCTS AND SERVICES

4 CELL BIOLOGY
4.7 Histology services - Immunohistochemistry (IHC)

Immunohistochemistry (IHC) is currently used in both research and clinical settings for the diagnosis of abnormal cells and the distribution and localization of certain proteins-of-interest in the cell. Gemini Biosciences offers IHC staining services using a wide range of validated antibodies - over 200 high specificity antibodies for a variety of pathological markers.

In addition to IHC staining, Gemini Bio’s full range of histology services includes tissue or cell pellet processing, embedding, sectioning and routine or special staining for paraffin samples.

 

Immunohistochemistry Staining Basic Service


(Primary antibody provided by customer)

 

Description

Immunohistochemistry staining of previously sectioned FFPE slides.

Customers to provide:
(1) a minimum of 20 μg of the primary antibody raised in either Mouse or Rabbit,
(2) information regarding the antigen retrieval method and optimal antibody working concentration for the marker of interest, 
(3) one positive control slide for the marker of interest.

Deliverables:

Cover-slipped stained IHC slide.

Immunohistochemistry Staining - Complete Service

(Primary antibody provided by Gemini Biosciences)
Immunohistochemistry staining of previously sectioned FFPE slides† with one of Gemini Biosciences repertoire of common antibodies. Please contact us for our list of validated IHC antibodies for this service.

Customers to provide
Test tissue blocks or slides for the marker of interest. 

Deliverables 

Cover-slipped stained IHC slide.

 

Immunohistochemistry Staining - Dual Staining Service

 

Immunohistochemistry staining of previously sectioned FFPE slides†. Dual staining service offers simultaneous staining of two markers of interest. This service is restricted to the simultaneous detection of one (1) Mouse antibody (Stained brown) and one (1) Rabbit antibody (Stained red).

Customers to provide:


(1) a minimum of 20 μg of each primary antibody
(2) information regarding optimal antibody dilution for both antibodies
(3) one positive control slide for each of the marker of interest.

Deliverables

Cover-slipped stained IHC slide.

 

†Please section your tissue at 4μm and mount them onto charged slides. 

Add-on services 


Have the tissue, but can’t embed it or cut it? Don’t worry, send us the tissue sample/block, and we can do the embedding and/or cutting for you!

Processing of Formalin-Fixed wet tissue suitable for paraffin embedding

Deliverables

Processed wet tissue suitable for paraffin embedding

 

Service options include:

 

  1. Paraffin Embedding

  2. Cell Pellet Processing and Embedding 

  3. Paraffin Sectioning 

  4. Serial Sectioning 

  5. Step Sectioning 

  6. Digital Scan 

  7. Image Analysis 

  8. Pathological Evaluation 

 

#Minimum order of 10 tissue samples is required.

 

Find us

Gemini Biosciences

131 Mount Pleasant

Liverpool Science Park

Liverpool

L3 5TF

United Kingdom

 

t: +44 (0) 151 230 2265 

e: orders@geminibioscience.com 

    info@geminibioscience.com 

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