PRODUCTS AND SERVICES
4 CELL BIOLOGY
4.10 Extracellular vesicles (EVs) analysis service
EVs are membrane-enclosed vesicles that can carry lipids, proteins, and RNAs, from one cell to another, for short or long distances. Depending on their size and origin, EVs can be characterized as exosomes (50–150nm in diameter), microvesicles (100nm–1μm in diameter) or large oncosomes (>1μm in diameter). Like Trojan horses, EVs can cross the cell barrier and reprogram cellular functions.
Gemini Biosciences offers a comprehensive and modular extracellular vesicles analysis service that involves production, purification and biochemical and biophysical analyses of the vesicles. Analysis techniques used include Nanoparticles Tracking Analysis (NTA) with the Nanosight NS300, fluorescence & electron microscopy, Western blotting, mass spectrometry (proteomics) and RNAseq.
We also offer a custom service involving the production, purification, and analysis of EVs from different cell lines subjected to various treatments according to the customer’s requirements. We have access to many human cancer and other cell lines available for use in customer experiments.
Please contact us for more information on cell lines available
We have developed an efficient workflow for the purification and characterization of extracellular vesicles from client samples including:
Cell culture supernatants
Cerebrospinal Fluid (CSF), etc.
Why are extracellular vesicles important?
EVs are involved in the intercellular communication by reciprocally shuttling signals and other molecules between same and/or different cell types. During the last few years, there has been intense research into their involvement in various biological processes including cell metabolism, proliferation, invasion, migration, immune responses and others as well as their contribution in tumour development and other diseases.
Potential applications of EVs
Considering their significance and multifaceted roles, EVs can be used as diagnostic and ultimately as therapeutic/clinical tools (i.e as biomarkers for the diagnosis and disease follow-up). Their clinical applications potential ranges from vaccination, improvement of pregnancy outcome and treatment of autoimmune diseases, cancer, and tissue injuries.