4.6 CRISPR-Cas9 cell engineering service

Gemini Biosciences provides a one-stop solution to all your gene editing services to engineer target cell lines for knock-out, knock-in, tagging, or single nucleotide modification applications.

KI confirmation by PCR or Sequencing


Basic Package Includes:


  1. Target DNA Vector Creation: gRNA Design and Construction (2-4 gRNAs); Donor DNA Construction (knock-in or point mutations)

  2. Cell Culture, Transfection, Optimization 

  3. Clonal Selection and Screening

  4. Clonal expansion and cryopreservation




  1. Genetically Engineered Cell Line (at least 1 clone, 2 vials per clone)

  2. Custom Report


*A deposit and MTA is required to initiate all CRISPR Cell Line projects


Disclaimer & MTA 


Orders of this service are subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at The end user acknowledges that the Materials provided under Gemini Biosciences' Material Transfer Agreement (MTA) does not grant a license for commercial use or imply any ownership rights of any intellectual property rights relating to the Materials.

Please note that if the gene to be knockout may be essential to cell survival, it is up to the end-user to proceed with the services. Gemini Biosciences is unable to guarantee cell survival in these cases and will only attempt to rescue the clones under these conditions. Gemini Biosciences is not accountable for cell survival if rescuing the clones (instructions to be provided customers) is unsuccessful. If the customer chooses to proceed, Gemini Biosciences will provide a hemizygous pool as the default deliverable to compensate for any lethal effects (unless otherwise requested)