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William Henry Duncan Building

6 West Derby Street

Liverpool

L7 8TX

United Kingdom

 

t: +44 (0) 151 794 9317 

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PRODUCTS AND SERVICES

2: CUSTOM ANTIBODY PRODUCTION
2.6 Antibody-based assay development

Using antibodies developed for the customer, Gemini Biosciences offers development of Two Antibody Sandwich Immunoassay Kits as part of its antibody production service and includes:

 

  • Development of monoclonal antibodies (MAb) against two different target epitopes (A and B) 
          

  • Production and purification of Ig from MAb A for capturing the antigen in ELISA test
     

  • Production and purification of Ig from MAb B for labeling of Ig with HRP for detection of captured antigen
     

  • Labeling of 1-3mg of purified Ig of MAb B with HRP or alkaline phosphatase
     

  • Testing of specificity of antibodies useful for ELISA
     

  • Evaluation of antibodies using target antigen in ELISA assay
     

  • Preparation of protocols and production of 10 ELISA kits