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7 BIOPHYSICAL ANALYSES
7.1 Isothermal titration calorimetry
Microcal ITC200 isothermal titration calorimeter used by Gemini Biosciences is the ‘gold standard’ for determining binding affinities.
The method will give information on the thermodynamics of binding, and the association constants. It is a method that is suitable for studying protein interactions with other proteins, peptides, nucleic acids and small molecules. The ITC method makes crucial contributions in the drug design process.
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