PRODUCTS AND SERVICES

6 STRUCTURAL BIOLOGY

6.1 Solution state biological NMR

Solution-state NMR is the most versatile analytical tool for the biophysical analysis of proteins.

 

Gemini Biosciences Process:

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Applications:

 

Solution-state NMR can be used for the following main purposes:

 

• determination of high- and atomic-resolution three-dimensional structures of proteins oof molecular mass smaller than about 30-35kD. 
   

• a quick assessment of whether  a recombinant protein is folded or unfolded, or in a molten globular state
   

• determination of whether and how a ligand binds to a protein. The ligand can be an inhibitor, natural ligand, cofactor, etc. The ligands can be small molecules, peptides, nucleic acids, carbohydrates, etc.
   

• determination of dissociation constants of weak binding ligands
   

• the initial screening  of compounds and fragments in a drug discovery campaign
   

• metabolite characterization

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Gemini Biosciences' NMR service includes the following:

 

• Designing appropriate approaches  and workflow for a structural biology project
   

• Expression and production of pure Escherichia coli expressed recombinant protein in milligram quantities
   

• Production of uniformly and selectively  13C, 15N, 2H- labeled proteins and peptides
   

• Acquisition of multi-dimensional NMR data for structure calculations
   

• Determination of protein structures by NMR and X-ray crystallography
   

• Determining the structures of proteins and ligands using a combination of NMR and computer docking.
   

• Sample preparation of tissues, cell extracts, and biofluids for metabolomics studies.

 

The following hardware is available for use to deliver our customers' projects:

 

• 800 MHz spectrometer with high sensitivity cryogenic heads for solution studies 

   

• 700 and 600 MHz spectrometers with high sensitivity cryogenic heads for solution studies plus automated sample changer for metabolomics, ligand screening, and metabolite identification

 

These three spectrometers are state-of-the-art and capable of performing most of the experiments that are essential for modern structural biology projects.

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Please contact us for details of our process and a quote.