PRODUCTS AND SERVICES

6 STRUCTURAL BIOLOGY
6.1 Solution state biological NMR

Solution-state NMR is the most versatile analytical tool for the biophysical analysis of proteins.

 

Gemini Biosciences Process:

 

 

 

 

 

 

 

 

Applications:

 

Solution-state NMR can be used for the following main purposes:

 

  • determination of high- and atomic-resolution three-dimensional structures of proteins oof molecular mass smaller than about 30-35kD. 
     

  • a quick assessment of whether  a recombinant protein is folded or unfolded, or in a molten globular state
     

  • determination of whether and how a ligand binds to a protein. The ligand can be an inhibitor, natural ligand, cofactor, etc. The ligands can be small molecules, peptides, nucleic acids, carbohydrates, etc.
     

  • determination of dissociation constants of weak binding ligands
     

  • the initial screening  of compounds and fragments in a drug discovery campaign
     

  • metabolite characterization

 

 

 

 

 

 

 

 

 

Gemini Biosciences' NMR service includes the following:

 

  • Designing appropriate approaches  and workflow for a structural biology project
     

  • Expression and production of pure Escherichia coli expressed recombinant protein in milligram quantities
     

  • Production of uniformly and selectively  13C, 15N, 2H- labeled proteins and peptides
     

  • Acquisition of multi-dimensional NMR data for structure calculations
     

  • Determination of protein structures by NMR and X-ray crystallography
     

  • Determining the structures of proteins and ligands using a combination of NMR and computer docking.
     

  • Sample preparation of tissues, cell extracts, and biofluids for metabolomics studies.

 

The following hardware is available for use to deliver our customers' projects:

 

  • 800 MHz spectrometer with high sensitivity cryogenic heads for solution studies 

   

  • 700 and 600 MHz spectrometers with high sensitivity cryogenic heads for solution studies plus automated sample changer for metabolomics, ligand screening, and metabolite identification

 

These three spectrometers are state-of-the-art and capable of performing most of the experiments that are essential for modern structural biology projects.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Please contact us for details of our process and a quote.

Find us

Liverpool Bio-Innovation Hub 

William Henry Duncan Building

6 West Derby Street

Liverpool

L7 8TX

United Kingdom

 

t: +44 (0) 151 794 9317 

e: orders@geminibioscience.com 

    info@geminibioscience.com 

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