4.5 CRISPR stable knockin cell line generation

Basic Package Includes:


  1. Target DNA Vector Creation; gRNA Design and Construction (2-4 gRNAs), Donor DNA Construction (knock-in or point mutation)

  2. Cell Culture, Transfection, Optimization

  3. Clonal Selection and Screening and KI Confirmation by PCR or Sequencing

  4. Clonal expansion and cryopreservation




  1. Genetically Engineered Cell Line (up to 2 vials 1x106 cells/vial)

  2. Custom Report


Add-On Services


  1. Cell Line of Your Choice for Gene Editing: 1 Cell Line

  2. WT Control Cell Line Expressing Cas9 for Comparison: 1 Cell Line

  3. Additional Clones: 1 Clone

  4. Additional Vials of Delivered Clones: 1 Vial

  5. Validation Service by Western Blot (Up to 10 Clones): 1 Service

  6. Off-Target Analysis by Whole Genome Sequencing: Per Clone

  7. Additional rounds of selection and screening by Sanger Sequencing: 1 Screening

  8. HA Tag Validation (Up to 10 Clones)#: 1 Service

  9. STR Profiling of WT and Knock-in cells: 1 Service

**A deposit and MTA is required to initiate all CRISPR Cell Line projects




Disclaimer & MTA 


Orders of this service are subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at The end user acknowledges that the Materials provided under Gemini Biosciences' Material Transfer Agreement (MTA) does not grant a license for commercial use or imply any ownership rights of any intellectual property rights relating to the Materials.

Please note that if the gene to be knockout may be essential to cell survival, it is up to the end-user to proceed with the services. Gemini Biosciences is unable to guarantee cell survival in these cases and will only attempt to rescue the clones under these conditions. Gemini Biosciences is not accountable for cell survival if rescuing the clones (instructions to be provided customers) is unsuccessful. If the customer chooses to proceed, Gemini Biosciences will provide a hemizygous pool as the default deliverable to compensate for any lethal effects (unless otherwise requested).